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KMID : 0379120010290010022
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Korean Journal of Mycology
2001 Volume.29 No. 1 p.22 ~ p.27
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Studies on Submerged Culture and Mycelial Components of Naematoloma sublateritium Mycelia
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Kang Tae-Su
Yoo Seung-Heon
Kang An-Seok
Cho Soo-Muk
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Abstract
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This study was carried out to get the basic information for the submerged culture and analyze the biochemical components of Naematoloma subdateritium mycelia. The optimal temperature, pH, agitation speed and cultural time for the mycelial growth of Naematoloma sublateritium were 25℃, 5.5, 150 rpm and 20 days, respectively. The proximate composition of mycelia was as follows; carbohydrate 55.8% (total sugar 48.7%), crude protein 22.4%, fat 4.1% and ash 4.7% respectively Among the free amino acid contents, phenylalanine, alanine and lysine were predominant component. The linoleic acid and pahnitic acid were found to be the highest among the free fatty acids. The biopolymer extracts of mycelia was identified to be protein-bounded polysaccharide by color reaction and sepharose CL-4B gel chromatography.
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